A comparative study of detection of p53 mutations in human breast cancer by flow cytometry, single-strand conformation polymorphism and genomic sequencing.

doi:10.1038/bjc.1996.514

Article

A comparative study of detection of p53 mutations in human breast cancer by flow cytometry, single-strand conformation polymorphism and genomic sequencing.

British journal of cancer - 1 Oct 1996

Chakravarty G, Redkar A, Mittra I

Abstract excerpt

The accuracy of immunodetection by dual parameter flow cytometry (FCM), polymerase chain reaction-mediated single strand conformation polymorphism (PCR-SSCP) and genomic sequencing to detect p53 mutations were compared. Analysis by the last two techniques was restricted to exons 5-8. Initially, 1...

Topics

Breast Neoplasms
Exons
Flow Cytometry
Fluorescent Antibody Technique
Genes, p53
Genome, Human
Humans
Immunohistochemistry
Mutation
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Tumor Suppressor Protein p53

A comparative study of detection of p53 mutations in human breast cancer by flow cytometry, single-strand conformation polymorphism and genomic sequencing.

Abstract excerpt

Topics

Discuss the evidence