Article
A novel flow cytometric method for the quantification of p53 gene expression.
Cytometry - 1 Mar 1998
Filippini G, Griffin S, Uhr M, Eppenberger H, Bonilla J, Cavalli F, Soldati G
Abstract excerpt
We describe a rapid and simple flow cytometry technique for the detection and quantification of p53 in several human cell lines, including an adenocarcinoma cell line (SW 626) having a mutant (m) p53, and a pre-B leukemia cell line (NALM-6) having wild-type (wt) p53. By introducing a second antibody coupled to RPE-fluorescence, the discrimination between control and specific peaks was improved over that achieved...
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