Human wild-type p53 adopts a unique conformational and phosphorylation state in vivo during growth arrest of glioblastoma cells.

Abstract excerpt

The wild-type (wt) human tumor-suppressor gene product, p53, and its mutant form have been analysed in an in vivo system in which the inducible expression of wt p53 results in growth arrest in the G1 phase of the cell cycle. Two major pools of p53 are detected after wt p53 expression by their differential reactivity with the p53 monoclonal antibodies PAb 421 and 1801 as well as the mutant and wt-specific...

Topics

• Base Sequence
• Electrophoresis, Gel, Two-Dimensional
• G1 Phase
• Glioma
• Humans
• Molecular Sequence Data
• Mutation
• Oligodeoxyribonucleotides
• Phosphorylation
• Polymerase Chain Reaction
• Precipitin Tests