Article
Reconstruction of the 2.4 Mb human DMD-gene by homologous YAC recombination.
Human molecular genetics - 1 Apr 1992
Den Dunnen J T, Grootscholten P M, Dauwerse J G, Walker A P, Monaco A P, Butler R, Anand R, Coffey A J, Bentley D R, Steensma H Y
Abstract excerpt
The human dystrophin gene, mutations of which cause Duchenne and Becker muscular dystrophy, measures 2.4 Mb. This size seriously limits its cloning as a single DNA fragment and subsequent in-vitro expression studies. We have used stepwise in-vivo recombination between overlapping yeast artificial chromosomes (YACs) to reconstruct the dystrophin gene. The recombinant YACs are mitotically stable upon propagation in...
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