Article
Reconstruction of the 2.4 Mb human DMD-gene by homologous YAC recombination.
Human molecular genetics - 1 Apr 1992
Den Dunnen J T, Grootscholten P M, Dauwerse J G, Walker A P, Monaco A P, Butler R, Anand R, Coffey A J, Bentley D R, Steensma H Y
Abstract excerpt
The human dystrophin gene, mutations of which cause Duchenne and Becker muscular dystrophy, measures 2.4 Mb. This size seriously limits its cloning as a single DNA fragment and subsequent in-vitro expression studies. We have used stepwise in-vivo recombination between overlapping yeast artificial...
Topics
- Chromosomes, Fungal
- Cloning, Molecular
- Crosses, Genetic
- Dystrophin
- Humans
- In Situ Hybridization
- Muscular Dystrophies
- Mutation
- Recombination, Genetic
- Saccharomyces cerevisiae
- X Chromosome