Article
Yeast kar1 mutants provide an effective method for YAC transfer to new hosts.
Genomics - 1 Jul 1994
Spencer F, Hugerat Y, Simchen G, Hurko O, Connelly C, Hieter P
Abstract excerpt
Yeast artificial chromosome (YAC) clones propagate large segments of exogenous DNA in a host organism with well-developed classical and molecular genetics. Most extant YAC clones are from libraries created in a single yeast host (AB1380). The application of techniques allowing the manipulation an...
Topics
- Chromosomes, Artificial, Yeast
- Cloning, Molecular
- Diploidy
- Gene Transfer Techniques
- Genes, Fungal
- Genetic Markers
- Genetic Vectors
- Haploidy
- Mutation
- Saccharomyces cerevisiae