Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Yeast kar1 mutants provide an effective method for YAC transfer to new hosts.

Genomics - 1 Jul 1994

Spencer F, Hugerat Y, Simchen G, Hurko O, Connelly C, Hieter P

Abstract excerpt

Yeast artificial chromosome (YAC) clones propagate large segments of exogenous DNA in a host organism with well-developed classical and molecular genetics. Most extant YAC clones are from libraries created in a single yeast host (AB1380). The application of techniques allowing the manipulation and/or restructuring of these cloned DNA segments often requires a change in the yeast genetic background to introduce...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cloning, Molecular
Share to Diploidy
Share to Gene Transfer Techniques

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genes, Fungal

Share to Genetic Markers

Share to Genetic Vectors

Share to Mutation

Share to Saccharomyces cerevisiae

Source

Identifiers

PMID: 7959757
DOI: 10.1006/geno.1994.1352

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Yeast kar1 mutants provide an effective method for YAC transfer to new hosts.PMID 7959757Read ↗

Transfer of YAC clones to new hosts by karyogamy-deficient mating.Semantic proximity Read ↗

A versatile method for efficient YAC transfer between any two strains.Semantic proximity Read ↗

Building larger YACs by recombination.Semantic proximity Read ↗

Enhanced mating-type switching and sexual hybridization in heterothallic yeast Yarrowia lipolytica.Semantic proximity Read ↗

Kar-mediated plasmid transfer between yeast strains: alternative to traditional transformation methods.Semantic proximity Read ↗

Chromosome-scale genetic mapping using a set of 16 conditionally stable Saccharomyces cerevisiae chromosomes.Semantic proximity Read ↗

Development of a highly efficient gene targeting system induced by transient repression of YKU80 expression in Candida glabrata.Semantic proximity Read ↗

Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.Semantic proximity Read ↗

Systematic hybrid LOH: a new method to reduce false positives and negatives during screening of yeast gene deletion libraries.Semantic proximity Read ↗

kem mutations affect nuclear fusion in Saccharomyces cerevisiae.Semantic proximity Read ↗

Rapid assembly of functional modules for generating human artificial chromosome constructs compatible with epigenetic centromere seedingSemantic proximity Read ↗

Different aneuploidies arise from the same bridge-induced chromosomal translocation event in Saccharomyces cerevisiae.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.