PCR amplification and analysis of yeast artificial chromosomes.

doi:10.1016/0888-7543(92)90051-s

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Article

PCR amplification and analysis of yeast artificial chromosomes.

Genomics - 1 Aug 1992

Sutcliffe J S, Zhang F, Caskey C T, Nelson D L, Warren S T

Abstract excerpt

A strategy for the analysis of yeast artificial chromosome (YAC) clones that relies on polymerase chain reaction (PCR) amplification of small restriction fragments from isolated YACs following adapter ligation was developed. Using this method, termed YACadapt, we have amplified several YACs from a human Xq24-qter library and have used the PCR products for physical mapping by somatic cell hybrid deletion analysis...

Topics

Animals
Base Sequence
Chromosomes, Fungal
Cricetinae
DNA
Electrophoresis, Agar Gel
Fragile X Syndrome
Gene Library
Genome, Human
Humans
Hybrid Cells
Karyotyping

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Male

Molecular Sequence Data

Mutation

Polymerase Chain Reaction

RNA

X Chromosome

Source

Open in PubMed

Identifiers

PMID: 1380485
DOI: 10.1016/0888-7543(92)90051-s

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1380485-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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