Article
The recovery of a B2 insertion in the lacI gene of a rat cell line containing a lambda/LIZ shuttle vector.
Mutation research - 14 Apr 1997
de Boer J G, Suri A, Walsh D, Glickman B W
Abstract excerpt
The bacterial lacI gene is in use as a reporter gene for mutation in transgenic mice, rats and in cell lines. During a mutagenesis study in which we used such a rat cell line, we recovered a mutant which features an insertion of a rat B2 repeated sequence element. This sequence element was insert...
Topics
- Animals
- Animals, Genetically Modified
- Bacterial Proteins
- Bacteriophage lambda
- Base Sequence
- Cell Line
- DNA Transposable Elements
- Escherichia coli Proteins
- Fibroblasts
- Genetic Vectors
- Lac Repressors
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Rats
- Repetitive Sequences, Nucleic Acid
- Repressor Proteins
- Sequence Alignment