Article
Vectors lambda 200g and lambda 200c: two useful derivatives of lambda 2001.
Gene - 16 Aug 1993
Koh C G, Barnett L, Brenner S
Abstract excerpt
We describe the construction and uses of two new phage lambda replacement vectors. Both are modifications of lambda 2001 which allows selection of cloned inserts by the Spi- phenotype, following the replacement of a gam+ filler fragment. Vector lambda 200g has a second gam gene with an amber mutation in its right arm which enables the selection of clones with inserts in the normal way in Su0 host bacteria and the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
