Vectors lambda 200g and lambda 200c: two useful derivatives of lambda 2001.

doi:10.1016/0378-1119(93)90353-5

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Article

Vectors lambda 200g and lambda 200c: two useful derivatives of lambda 2001.

Gene - 16 Aug 1993

Koh C G, Barnett L, Brenner S

Abstract excerpt

We describe the construction and uses of two new phage lambda replacement vectors. Both are modifications of lambda 2001 which allows selection of cloned inserts by the Spi- phenotype, following the replacement of a gam+ filler fragment. Vector lambda 200g has a second gam gene with an amber muta...

Topics

Attachment Sites, Microbiological
Bacteriophage lambda
Chloramphenicol O-Acetyltransferase
Cloning, Molecular
DNA Restriction Enzymes
DNA, Recombinant
Genes, Suppressor
Genes, Viral
Genetic Complementation Test
Genetic Vectors
Mutation
Suppression, Genetic

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