Article
An HMG I/Y-containing repressor complex and supercoiled DNA topology are critical for long-range enhancer-dependent transcription in vitro.
Genes & development - 1 Mar 1997
Bagga R, Emerson B M
Abstract excerpt
The 3' enhancer of the T cell receptor alpha-chain (TCR alpha) gene directs the tissue- and stage-specific expression and V(D)J recombination of this gene locus. Using an in vitro system that reproduces TCR alpha enhancer activity efficiently, we show that long-range promoter-enhancer regulation...
Topics
- DNA
- DNA-Binding Proteins
- Enhancer Elements, Genetic
- HMGA1a Protein
- High Mobility Group Proteins
- Humans
- Jurkat Cells
- Lymphoid Enhancer-Binding Factor 1
- Mutation
- Nucleic Acid Conformation
- Promoter Regions, Genetic
- RNA Polymerase II
- Receptors, Antigen, T-Cell
- Receptors, Antigen, T-Cell, alpha-beta
- Repressor Proteins
- Transcription Factors
- Transcription, Genetic