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Article

Variant cDNA sequences of human ATP:citrate lyase: cloning, expression, and purification from baculovirus-infected insect cells.

Protein expression and purification - 1 Feb 1997

Lord K A, Wang X M, Simmons S J, Bruckner R C, Loscig J, O'Connor B, Bentley R, Smallwood A, Chadwick C C, Stevis P E, Ciccarelli R B

Abstract excerpt

ATP:citrate lyase (ACL) is a major generator of cytosolic acetyl-coenzymeA, which is required for both fatty acid and cholesterol biosynthesis. The human ACL (hACL) cDNA was cloned by RT-PCR, and our results indicate the existence of previously unknown sequence variations in hACL. Expression of the hACL cDNA in Spodoptera frugiperda 9 insect cells resulted in the production of high levels of soluble, active...

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PMID: 9116495
DOI: 10.1006/prep.1996.0668

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