Article
Critical role of glutamine 252 in the hormone-dependent transcriptional activity of the thyroid hormone beta1 nuclear receptor.
Biochemistry - 8 Apr 1997
Bhat M K, McPhie P, Cheng S
Abstract excerpt
To understand the molecular basis of the ligand-dependent transcriptional activity of thyroid hormone nuclear receptors (TRs), we investigated the effect of mutation of glutamine 252 (Q252) on the function of human TR subtype beta1 (wTRbeta1). Q252 is conserved in TRs in all species and is located in a region of the hormone binding domain that has been shown to undergo 3,3',5-triiodo-L-thyronine (T3) induced...
Topics
- Antibodies, Monoclonal
- Binding Sites
- Binding, Competitive
- Blotting, Western
- Cloning, Molecular
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Genes, Reporter
- Glutamine
- Humans
- Mutation