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Article

Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins.

Nature protocols - 1 Jan 2007

Guan Lan, Kaback H Ronald

Abstract excerpt

This protocol describes a detailed method to study the static and dynamic features of membrane proteins, as well as solvent accessibility, by utilizing the lactose permease of Escherichia coli (LacY) as a model. The method relies on the use of functional single-Cys mutants, an affinity tag and a PhosphoImager. The membrane-permeant, radioactive thiol reagent N-[ethyl-1-14C]ethylmaleimide ([14C]NEM) is used to...

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Identifiers

PMID: 17703213
DOI: 10.1038/nprot.2007.275
PMCID: PMC2775809

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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