Article
Insertion of the mannitol permease into the membrane of Escherichia coli. Possible involvement of an N-terminal amphiphilic sequence.
The Journal of biological chemistry - 25 Sept 1991
Yamada Y, Chang Y Y, Daniels G A, Wu L F, Tomich J M, Yamada M, Saier M H
Abstract excerpt
The in vivo membrane assembly of the mannitol permease, the mannitol Enzyme II (IImtl) of the Escherichia coli phosphotransferase system, has been studied employing molecular genetic approaches. Removal of the N-terminal amphiphilic leader of the permease and replacement with a short hydrophobic sequence resulted in an inactive protein unable to transport mannitol into the cell or catalyze either...
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