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Article

Biosynthesis, purification, and characterization of the human coronavirus 229E 3C-like proteinase

1 May 1997

Abstract excerpt

Coronavirus gene expression involves proteolytic processing of the gene 1-encoded polyprotein(s), and a key enzyme in this process is the viral 3C-like proteinase. In this report, we describe the biosynthesis of the human coronavirus 229E 3C-like proteinase in Escherichia coli and the enzymatic properties, inhibitor profile, and substrate specificity of the purified protein. Furthermore, we have introduced single...

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Identifiers

PMID: 9094676
DOI: 10.1128/jvi.71.5.3992-3997.1997

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