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Article

A novel auto-cleavage assay for studying mutational effects on the active site of severe acute respiratory syndrome coronavirus 3C-like protease.

Biochemical and biophysical research communications - 12 Nov 2004

Shan Yu-Fei, Li Shou-Feng, Xu Gen-Jun

Abstract excerpt

The 3C-like protease (3CLpro) of severe acute respiratory syndrome (SARS) has been proposed as an attractive target for drug design. His41 and Cys145 were essential for the active site as the principal catalytic residues. In this study, we mutated the two sites, expressed four resulting mutants in Escherichia coli and characterized. All mutants showed undetectable activity in trans-cleavage assay. In addition, we...

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Identifiers

PMID: 15474466
DOI: 10.1016/j.bbrc.2004.09.088
PMCID: PMC7092848

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Source receipt: pubmed, retrieved 7 Sept 2026.

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