Article
Site-directed mutagenesis suggests close functional relationship between a human rhinovirus 3C cysteine protease and cellular trypsin-like serine proteases.
The Journal of biological chemistry - 5 May 1990
Cheah K C, Leong L E, Porter A G
Abstract excerpt
Human rhinoviruses, like other picornaviruses, encode a cysteine protease (designated 3C) which cleaves mainly at viral Gln-Gly pairs. There are significant areas of homology between picornavirus 3C cysteine proteases and cellular serine proteases (e.g. trypsin), suggesting a functional relationship between their catalytic regions. To test this functional relationship, we made single substitutions in human...
Topics
- Amino Acid Sequence
- Base Sequence
- Cysteine Endopeptidases
- Humans
- Immune Sera
- Immunoblotting
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Peptides
- Plasmids