Reliable method for generating double-stranded DNA vectors containing site-specific base modifications.

doi:10.2144/04375ST01

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Article

Reliable method for generating double-stranded DNA vectors containing site-specific base modifications.

BioTechniques - 1 Nov 2004

Brégeon Damien, Doetsch Paul W

Abstract excerpt

Cells of all living organisms are continuously exposed to physical and chemical agents that damage DNA and alter the integrity of their genomes. Despite the relatively high efficiency of the different repair pathways, some lesions remain in DNA when it is replicated or transcribed. Lesion bypass by DNA and RNA polymerases has been the subject of numerous investigations. However, knowledge of the in vivo mechanism...

Topics

Base Pair Mismatch
DNA
DNA Damage
Genetic Engineering
Genetic Vectors
Mutagenesis, Site-Directed
Mutation
Reproducibility of Results

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Open in PubMed

Identifiers

PMID: 15560132
DOI: 10.2144/04375ST01

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