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Article

Relationship between thermal stability, degradation rate and expression yield of barnase variants in the periplasm of Escherichia coli.

Protein engineering - 1 Dec 1996

Kwon W S, Da Silva N A, Kellis J T

Abstract excerpt

An advantage of exporting a recombinant protein to the periplasm of Escherichia coli is decreased proteolysis in the periplasm compared with that in the cytoplasm. However, protein degradation in the periplasm also occurs. It has been widely accepted that the thermodynamic stability of a protein is an important factor for protein degradation in the cytoplasm of E.coli. To investigate the effect of the...

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Identifiers

PMID: 9010933
DOI: 10.1093/protein/9.12.1197

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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