Article
Secretory expression of a heterologous protein, Aiio-AIO6BS, in Bacillus subtilis via a non-classical secretion pathway.
Biochemical and biophysical research communications - 16 Sept 2016
Pan Xingliang, Yang Yalin, Liu Xuewei, Li Dong, Li Juan, Guo Xiaoze, Zhou Zhigang
Abstract excerpt
The quenching enzyme AIO6 (AiiO-AIO6) has been reported as a feed additive preparation for application in aquaculture and biological control of pathogenic Aeromonas hydrophila. We developed an economical strategy to express AIO6BS (AiiO-AIO6BS, codon optimized AIO6 in Bacillus subtilis) in Bacillus subtilis for facilitating its widespread application. Promoter p43 without the signal peptide was used for secretory...
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