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Article

[I87E mutation prevents barstar dimerization].

Bioorganicheskaia khimiia - 1 Jan 2000

Korchuganov D S, Shul'ga A A, Ermoliuk Ia S, Mit'kevich V A, Reĭbarkh M Ia, Nol'de S B, Makarov A A, Arsen'ev A S, Kirpichnikov M P

Abstract excerpt

The C40,82A;I87E mutant of barstar, an intracellular inhibitor of the ribonuclease barnase from Bacillus amyloliquefaciens, was obtained, and its physicochemical properties were studied. It was produced as a fusion protein with thioredoxin and then cleaved from this by EKmax enterokinase. The mutant was shown by NMR to retain the spatial structure of the wild-type protein but, in contrast to barstar, does not...

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Identifiers

PMID: 15586816
DOI: 10.1023/b:rubi.0000049775.11808.a1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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