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Article

Overproduction and purification of Lon protease from Escherichia coli using a maltose-binding protein fusion system.

Applied microbiology and biotechnology - 1 Nov 1994

Sonezaki S, Kondo A, Oba T, Ishii Y, Kato Y, Nakayama H

Abstract excerpt

Lon protease, which plays a major role in degradation of abnormal proteins in Escherichia coli, was overproduced and efficiently purified using the maltose-binding protein (MBP) fusion vector. The MBP-Lon fusion protein was expressed in a soluble form in E. coli and purified to homogeneity by amylose resin in a single step. Lon protease was split from MBP by cleaving a fusion point between MBP and Lon with factor...

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Identifiers

PMID: 7765772
DOI: 10.1007/BF00902735

Genes

MBP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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