Article
The clustering of telomeres and colocalization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae.
The Journal of cell biology - 1 Sept 1996
Gotta M, Laroche T, Formenton A, Maillet L, Scherthan H, Gasser S M
Abstract excerpt
We have developed a novel technique for combined immunofluorescence/in situ hybridization on fixed budding yeast cells that maintains the three-dimensional structure of the nucleus as monitored by focal sections of cells labeled with fluorescent probes and by staining with a nuclear pore antibody...
Topics
- Antibody Specificity
- Blotting, Western
- Cell Nucleus
- Fluorescent Antibody Technique
- Fungal Proteins
- GTP-Binding Proteins
- In Situ Hybridization, Fluorescence
- Mutation
- RNA, Messenger
- Saccharomyces cerevisiae
- Silent Information Regulator Proteins, Saccharomyces cerevisiae
- Telomere
- Trans-Activators
- Transcription Factors
