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Article

Ligase chain reaction assay for human mutations: the Sickle Cell by LCR assay.

Clinical chemistry - 1 Jan 1997

Reyes A A, Carrera P, Cardillo E, Ugozzoli L, Lowery J D, Lin C I, Go M, Ferrari M, Wallace R B

Abstract excerpt

We can detect the beta-globin gene sickle cell mutation by using an assay based on the ligase chain reaction. The simultaneous amplification of the human growth hormone gene in the same reaction serves as a control for the amount of template DNA or amplification efficiency. Ligation products, which are biotinylated at one end and tagged with an arbitrary "tail" sequence at the other, are captured by hybridization...

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PMID: 8990220

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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