Article
Ligase chain reaction assay for human mutations: the Sickle Cell by LCR assay.
Clinical chemistry - 1 Jan 1997
Reyes A A, Carrera P, Cardillo E, Ugozzoli L, Lowery J D, Lin C I, Go M, Ferrari M, Wallace R B
Abstract excerpt
We can detect the beta-globin gene sickle cell mutation by using an assay based on the ligase chain reaction. The simultaneous amplification of the human growth hormone gene in the same reaction serves as a control for the amount of template DNA or amplification efficiency. Ligation products, whi...
Topics
- Alleles
- Anemia, Sickle Cell
- Colorimetry
- DNA
- DNA Ligases
- Genotype
- Globins
- Hemoglobin, Sickle
- Human Growth Hormone
- Humans
- Mutation
- Oligonucleotides