Article
Simultaneous analysis of mutant and normal alleles for multiple cystic fibrosis mutations by the ligase chain reaction.
Human mutation - 1 Jan 1995
Fang P, Bouma S, Jou C, Gordon J, Beaudet A L
Abstract excerpt
The ligase chain reaction (LCR) involves repetitive cycles of ligation of two adjacent pairs of oligonucleotides to form longer ligated products in a template-dependent manner. This study demonstrates the application of LCR for analysis of multiple small mutations. We adapted the technology for the simultaneous determination of the normal and mutant alleles in a competition format, as well as multiple mutations...
Topics
- Alleles
- Base Sequence
- Chromosome Mapping
- Cystic Fibrosis
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction