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Article

High accuracy genotyping directly from genomic DNA using a rolling circle amplification based assay.

BMC genomics - 30 May 2003

Alsmadi Osama A, Bornarth Carole J, Song Wanmin, Wisniewski Michele, Du Jing, Brockman Joel P, Faruqi A Fawad, Hosono Seiyu, Sun Zhenyu, Du Yuefen, Wu Xiaohong, Egholm Michael, Abarzúa Patricio, Lasken Roger S, Driscoll Mark D

Abstract excerpt

BACKGROUND: Rolling circle amplification of ligated probes is a simple and sensitive means for genotyping directly from genomic DNA. SNPs and mutations are interrogated with open circle probes (OCP) that can be circularized by DNA ligase when the probe matches the genotype. An amplified detection signal is generated by exponential rolling circle amplification (ERCA) of the circularized probe. The low cost and...

Topics

Chromosomes, Human, Pair 6
DNA
DNA Probes
DNA, Circular
Factor V
Female
Genotype
HLA Antigens
Humans
Mutation
Nucleic Acid Amplification Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleic Acid Conformation

Point Mutation

Prothrombin

Retrospective Studies

Source

Open in PubMed Open full text

Identifiers

PMID: 12777185
DOI: 10.1186/1471-2164-4-21
PMCID: PMC165428

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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