Article
A new genotyping method for detecting low abundance single nucleotide mutations based on gap ligase chain reaction and quantitative PCR assay.
Cell biochemistry and biophysics - 1 Jan 2012
Yi Ping, Jiang Hongmei, Li Li, Dai Faguo, Zheng Yingru, Han Jian, Chen Zhuqin, Guo Jianxin
Abstract excerpt
We tested applicability of a new genotyping technique to detect a low abundance CD17 (A → T) mutation of β-globin gene. The technique utilized a combined gap ligase chain reaction (Gap-LCR) and quantitative PCR (qPCR) methods. One pair of Gap-LCR primers was modified by adding specific sequences to the 5' end of the upstream and the 3' end of the downstream primer which served as a combining sequence for qPCR....
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