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Article

Evaluation of the ligase chain reaction (LCR) for the detection of point mutations.

Mutation research - 1 Oct 1992

Kälin I, Shephard S, Candrian U

Abstract excerpt

The ligase chain reaction (LCR) was evaluated as an amplification method for an in vivo mutation assay. Specifically, the ligase was tested for its ability to selectively amplify a DNA sequence mutated at a single base, in the presence of an excess of wild-type DNA. As a model template a 370-bp DNA fragment of the mouse Ha-ras protooncogene containing an A to T mutation at the second position of codon 61 was...

Topics

Animals
Base Sequence
Blotting, Southern
Cloning, Molecular
DNA
DNA Ligases
Electrophoresis, Polyacrylamide Gel
Evaluation Studies as Topic
Genes, ras
Ligases
Mice
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenicity Tests

Mutation

Nucleic Acid Amplification Techniques

Polymerase Chain Reaction

Templates, Genetic

Source

Identifiers

PMID: 1381488
DOI: 10.1016/0165-7992(92)90143-6

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1381488-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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