Article
A new approach for point mutation detection based on a ligase chain reaction.
Journal of biochemical and biophysical methods - 4 Dec 2001
Demchinskaya A V, Shilov I A, Karyagina A S, Lunin V G, Sergienko O V, Voronina O L, Leiser M, Plobner L
Abstract excerpt
A new method for the identification of point mutations is proposed. The method is based on ligase chain reaction (LCR) and it includes a procedure for correction of ligation by Cleavase. Reaction products are detected by a colorimetric method after adsorption of the resulting DNA duplexes to the solid phase. One strand of LCR products carries biotin to be bound on a streptavidin-coated microwell. Another strand...
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