Article
Method of obtaining mutants defective in immunoglobulin mu transcription factors.
Bioscience, biotechnology, and biochemistry - 1 Nov 1996
Shindo T, Ueda H, Makishima F, Nishimura H, Suzuki E
Abstract excerpt
A method of obtaining mutants defective in the regulatory function of Ig mu gene expression was developed. Such mutants are useful for discovering the functions of transcription factors and isolating their genes, especially those of DNA-unbinding factors. Cells expressing Ig mu and Eco-GPT simult...
Topics
- 3T3 Cells
- Animals
- Cell Fusion
- Cells, Cultured
- Flow Cytometry
- Hypoxanthine Phosphoribosyltransferase
- Immunoglobulin M
- Mice
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Transcription Factors
- Transfection
