Neighboring plasmid sequences can affect Mini-Mu DNA transposition in the absence of expression of the bacteriophage Mu semi-essential early region.

Abstract excerpt

Bacteriophage Mu DNA, like other transposable elements, requires DNA sequences at both extremities to transpose. It has been previously demonstrated that the transposition activity of various transposons can be influenced by sequences outside their ends. We have found that alterations in the neighboring plasmid sequences near the right extremity of a Mini-Mu, inserted in the plasmid pSC101, can exert an influence...

Topics

• Bacteriophage mu
• Base Sequence
• DNA Transposable Elements
• Escherichia coli
• Gene Deletion
• Molecular Sequence Data
• Mutation
• Plasmids
• Promoter Regions, Genetic
• Transcription, Genetic