Article
[The transformation of the unicellular alga Chlamydomonas reinhardtii by electroporation].
Mikrobiologiia - 1 Jan 2000
Ladygin V G
Abstract excerpt
The cell wall-lacking mutant CW-15 of the unicellular green alga Chlamydomonas reinhardtii was transformed by electroporation using plasmid pCTVHyg, which was constructed with the hygromycin phosphotransferase gene hpt as the selective marker and the Tn5 transposon of Escherichia coli under the control of the virus SV40 early gene promoter. Under optimal conditions (10(6) mid-exponential cells/ml; electric field...
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