Article
Rapid construction and screening of artificial microRNA systems in Chlamydomonas reinhardtii.
The Plant journal : for cell and molecular biology - 1 Sept 2014
Hu Jinlu, Deng Xuan, Shao Ning, Wang Gaohong, Huang Kaiyao
Abstract excerpt
The unicellular green algae Chlamydomonas reinhardtii is a classic model for the study of flagella/cilia and photosynthesis, and it has recently been exploited for producing biopharmaceuticals and biofuel. Due to the low frequency of homologous recombination, reverse genetic manipulation in Chlamydomonas relies mainly on miRNA- and siRNA-based knockdown methods. However, the difficulty in constructing artificial...
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