Article
High-frequency nuclear transformation of Chlamydomonas reinhardtii.
Proceedings of the National Academy of Sciences of the United States of America - 1 Feb 1990
Kindle K L
Abstract excerpt
By using a method in which cell-wall-deficient Chlamydomonas reinhardtii cells were agitated in the presence of DNA, glass beads, and polyethylene glycol, nuclear transformation rates of approximately 10(3) transformants per micrograms of plasmid DNA were achieved. The nitrate reductase gene from wild-type Chlamydomonas was used to complement a mutation in the corresponding gene of a strain containing nit1-305....
Topics
- Cell Nucleus
- Chlamydomonas
- Chlorides
- Cobalt
- Dimethyl Sulfoxide
- Glass
- Indicators and Reagents
- Kinetics
- Mannitol
- Mutation
- Plasmids
- Polyamines