Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

[Use of the hygromycin phosphotransferase gene as the dominant selective marker for Chlamydomonas reinhardtii transformation].

Molekuliarnaia biologiia - 1 Jan 2000

Butanaev A M

Abstract excerpt

The hygromycin phosphotransferase gene (hpt) from E. coli under the control of the SV40 early promoter was used as a dominant selectable marker for transformation of Chlamydomonas reinhardtii. Cells were transformed by electroporation (pulse length, 2 ms, field strength, 1 kV/cm). The culture gro...

Topics

Animals
Cell Nucleus
Chlamydomonas reinhardtii
Cloning, Molecular
Codon
Electroporation
Escherichia coli
Genes, Dominant
Genetic Markers
Phenotype
Phosphotransferases (Alcohol Group Acceptor)
Promoter Regions, Genetic

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Simian virus 40

Transformation, Genetic

Source

Identifiers

PMID: 7990829

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7990829-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

[Use of the hygromycin phosphotransferase gene as the dominant selective marker for Chlamydomonas reinhardtii transformation].PMID 7990829Read ↗

[Transformation of Chlamydomonas reinhardtii CW-15 with the hygromycin phosphotransferase gene as a selective marker].Semantic proximity Read ↗

[The transformation of the unicellular alga Chlamydomonas reinhardtii by electroporation].Semantic proximity Read ↗

Stable nuclear transformation of Chlamydomonas reinhardtii with a Streptomyces rimosus gene as the selective marker.Semantic proximity Read ↗

In vivo directed evolution for thermostabilization of Escherichia coli hygromycin B phosphotransferase and the use of the gene as a selection marker in the host-vector system of Thermus thermophilus.Semantic proximity Read ↗

[The green alga Chlamydomonas reinhardtii as an attractive model system].Semantic proximity Read ↗

Stable nuclear transformation of Chlamydomonas reinhardtii by using a C. reinhardtii gene as the selectable marker.Semantic proximity Read ↗

Effect of the selective medium on the manifestation of mutations induced with mono-alkylating agents in Chlamydomonas reinhardi.Semantic proximity Read ↗

Plant genetic transformation efficiency of selected Malaysian rice based on selectable marker gene (hptII).Semantic proximity Read ↗

High-frequency nuclear transformation of Chlamydomonas reinhardtii.Semantic proximity Read ↗

[Precise excision of TN5 in Escherichia coli K12 mutants with alterations in common component of phosphotransferase system and in subunits of DNA-gyrase].Semantic proximity Read ↗

Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker.Semantic proximity Read ↗

Molecular analysis of the acetolactate synthase gene of Chlamydomonas reinhardtii and development of a genetically engineered gene as a dominant selectable marker for genetic transformation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.