Article
Functional genomics of the regulation of the nitrate assimilation pathway in Chlamydomonas.
Plant physiology - 1 Feb 2005
González-Ballester David, de Montaigu Amaury, Higuera José Javier, Galván Aurora, Fernández Emilio
Abstract excerpt
The existence of mutants at specific steps in a pathway is a valuable tool of functional genomics in an organism. Heterologous integration occurring during transformation with a selectable marker in Chlamydomonas (Chlamydomonas reinhardtii) has been used to generate an ordered mutant library. A strain, having a chimeric construct (pNia1::arylsulfatase gene) as a sensor of the Nia1 gene promoter activity, was...
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