Article
A transcriptionally active estrogen receptor mutant is a novel type of dominant negative inhibitor of estrogen action.
Molecular endocrinology (Baltimore, Md.) - 1 Dec 1996
McInerney E M, Ince B A, Shapiro D J, Katzenellenbogen B S
Abstract excerpt
We have characterized a human estrogen receptor (ER) mutant, V364E, which has a single amino acid substitution in its hormone-binding domain. This ER mutant is fully active or even superactive at saturating levels of estradiol (10(-8) M E2) yet has the capacity to act as a strong dominant negativ...
Topics
- Animals
- Binding Sites
- Binding, Competitive
- CHO Cells
- Cricetinae
- Estrogens
- Mutation
- Point Mutation
- Promoter Regions, Genetic
- Receptors, Estrogen
- Recombinant Proteins
- Repressor Proteins