Article
Characterization of a Tn5 pre-cleavage synaptic complex.
Journal of molecular biology - 8 Sept 2000
Bhasin A, Goryshin I Y, Steiniger-White M, York D, Reznikoff W S
Abstract excerpt
Protein catalyzed DNA rearrangements typically require assembly of complex nucleoprotein structures. In transposition and integration reactions, these structures, termed synaptic complexes, are mandatory for catalysis. We characterize the Tn5 pre-cleavage synaptic complex, the simplest transposition complex described to date. We identified this complex by gel retardation assay using short, linear fragments and...
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