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Article

Targeted disruption of the mouse topoisomerase I gene by camptothecin selection.

Molecular and cellular biology - 1 Dec 1996

Morham S G, Kluckman K D, Voulomanos N, Smithies O

Abstract excerpt

Topoisomerase I has ubiquitous roles in important cellular functions such as replication, transcription, and recombination. In order to further characterize this enzyme in vivo, we have used gene targeting to inactivate the mouse Top-1 gene. A selection protocol using the topoisomerase I inhibitor camptothecin facilitated isolation of embryonic stem cell clones containing an inactivated allele; isolation of...

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Identifiers

PMID: 8943335
DOI: 10.1128/MCB.16.12.6804
PMCID: PMC231683

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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