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Article

Studying vertebrate topoisomerase 2 function using a conditional knockdown system in DT40 cells.

Nucleic acids research - 1 Aug 2009

Johnson Mark, Phua Hui Hui, Bennett Sophia C, Spence Jennifer M, Farr Christine J

Abstract excerpt

DT40 is a B-cell lymphoma-derived avian cell line widely used to study cell autonomous gene function because of the high rates with which DNA constructs are homologously recombined into its genome. Here, we demonstrate that the power of the DT40 system can be extended yet further through the use of RNA interference as an alternative to gene targeting. We have generated and characterized stable DT40 transfectants...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 19494182
DOI: 10.1093/nar/gkp480
PMCID: PMC2724289

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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