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Article

Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype.

Nature biotechnology - 1 May 2003

Kunath Tilo, Gish Gerald, Lickert Heiko, Jones Nina, Pawson Tony, Rossant Janet

Abstract excerpt

Gene targeting via homologous recombination in murine embryonic stem (ES) cells has been the method of choice for deciphering mammalian gene function in vivo. Despite improvements in this technology, it still remains a laborious method. Recent advances in RNA interference (RNAi) technology have provided a rapid loss-of-function method for assessing gene function in a number of organisms. Studies in mammalian cell...

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Identifiers

PMID: 12679785
DOI: 10.1038/nbt813

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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