Article
Characterization of new vacuolar segregation mutants, isolated by screening for loss of proteinase B self-activation.
European journal of cell biology - 1 Nov 1996
Gomes de Mesquita D S, van den Hazel H B, Bouwman J, Woldringh C L
Abstract excerpt
Part of the vacuole in the mother cell of Saccharomyces cerevisiae is segregated early in the cell cycle to establish a new vacuole in the bud. Investigation of the molecular mechanism of vacuolar segregation has previously been limited by the lack of an efficient screen for mutants defective in...
Topics
- Aspartic Acid Endopeptidases
- Carboxypeptidases
- Cathepsin A
- Enzyme Activation
- Microscopy, Fluorescence
- Microscopy, Phase-Contrast
- Mutagenesis
- Phenotype
- Saccharomyces cerevisiae
- Serine Endopeptidases
- Vacuoles
- Zygote
