Characterization of new vacuolar segregation mutants, isolated by screening for loss of proteinase B self-activation.

Abstract excerpt

Part of the vacuole in the mother cell of Saccharomyces cerevisiae is segregated early in the cell cycle to establish a new vacuole in the bud. Investigation of the molecular mechanism of vacuolar segregation has previously been limited by the lack of an efficient screen for mutants defective in this process. We developed a new screening procedure based on a cascade for activation of vacuolar proteases....

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