Article
Autoactivation of proteinase A initiates activation of yeast vacuolar zymogens.
European journal of biochemistry - 1 Jul 1992
van den Hazel H B, Kielland-Brandt M C, Winther J R
Abstract excerpt
The Saccharomyces cerevisiae PEP4 gene encodes proteinase A, an aspartyl protease. pep4 mutants are defective in the activation of many vacuolar hydrolases, including proteinase B. We have expressed a pep4 mutation which directs the accumulation of pro-proteinase A with a defective active site. Co-expression with PEP4 leads to normal processing, i.e. the mutant zymogen is functional as a substrate for the...
Topics
- Alleles
- Amino Acid Sequence
- Aspartic Acid Endopeptidases
- Base Sequence
- Binding Sites
- Codon
- Enzyme Activation
- Enzyme Precursors
- Genes, Fungal
- Molecular Sequence Data
- Mutagenesis, Site-Directed