Article
Dual effect of a Tn917 insertion into the Bacillus subtilis sacX gene.
Journal of general microbiology - 1 Jan 1991
Le Coq D, Aymerich S, Steinmetz M
Abstract excerpt
The most common effect of transposon insertion is the inactivation of genes. However, in some cases, transposons can activate in cis the expression of genes in the neighbourhood of their integration site. We previously described an insertion of the transposon Tn917 into the Bacillus subtilis sacXY locus. sacX and sacY encode respectively a negative and a positive regulator involved in induction by sucrose of the...
Topics
- Bacillus subtilis
- Cloning, Molecular
- DNA Transposable Elements
- Gene Expression Regulation
- Genes, Bacterial
- Mutation
- Recombination, Genetic
- Restriction Mapping
- Transcription, Genetic
