Selected mutations in Bacillus subtilis levansucrase semi-conserved regions affecting its biochemical properties.

doi:10.1093/protein/gzn036

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Article

Selected mutations in Bacillus subtilis levansucrase semi-conserved regions affecting its biochemical properties.

Protein engineering, design & selection : PEDS - 1 Oct 2008

Ortiz-Soto Maria Elena, Rivera Manuel, Rudiño-Piñera Enrique, Olvera Clarita, López-Munguía Agustin

Abstract excerpt

Levansucrases (LS) are fructosyltransferases (FTFs) belonging to family 68 of glycoside hydrolases (GH68) using sucrose as substrate to synthesize levan, a fructose polymer. From a multiple sequence analysis of GH68 family proteins, nine residues were selected and their role in acceptor and product specificity, as well as in biochemical Bacillus subtilis LS properties, was investigated. A product specificity...

Topics

Bacillus subtilis
Base Sequence
Conserved Sequence
Crystallography, X-Ray
Enzyme Activation
Fructans
Hexosyltransferases
Kinetics
Molecular Weight
Mutant Proteins
Mutation
Substrate Specificity

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Open in PubMed

Identifiers

PMID: 18596022
DOI: 10.1093/protein/gzn036

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