Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Selected mutations in Bacillus subtilis levansucrase semi-conserved regions affecting its biochemical properties.

Protein engineering, design & selection : PEDS - 1 Oct 2008

Ortiz-Soto Maria Elena, Rivera Manuel, Rudiño-Piñera Enrique, Olvera Clarita, López-Munguía Agustin

Abstract excerpt

Levansucrases (LS) are fructosyltransferases (FTFs) belonging to family 68 of glycoside hydrolases (GH68) using sucrose as substrate to synthesize levan, a fructose polymer. From a multiple sequence analysis of GH68 family proteins, nine residues were selected and their role in acceptor and product specificity, as well as in biochemical Bacillus subtilis LS properties, was investigated. A product specificity...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacillus subtilis
Share to Conserved Sequence

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Crystallography, X-Ray

Share to Enzyme Activation

Share to Hexosyltransferases

Share to Molecular Weight

Share to Mutant Proteins

Share to Substrate Specificity

Source

Identifiers

PMID: 18596022
DOI: 10.1093/protein/gzn036

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Selected mutations in Bacillus subtilis levansucrase semi-conserved regions affecting its biochemical properties.PMID 18596022Read ↗

Implications of the mutation S164A on Bacillus subtilis levansucrase product specificity and insights into protein interactions acting upon levan synthesis.Semantic proximity Read ↗

Polymerase and hydrolase activities of Bacillus subtilis levansucrase can be separately modulated by site-directed mutagenesis.Semantic proximity Read ↗

Computational design of oligosaccharide producing levansucrase from Bacillus licheniformis RN-01 to improve its thermostability for production of levan-type fructooligosaccharides from sucrose.Semantic proximity Read ↗

Donor substrate recognition in the raffinose-bound E342A mutant of fructosyltransferase Bacillus subtilis levansucrase.Semantic proximity Read ↗

Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution.Semantic proximity Read ↗

Probing impact of active site residue mutations on stability and activity of Neisseria polysaccharea amylosucrase.Semantic proximity Read ↗

Bacillus subtilis 168 levansucrase (SacB) activity affects average levan molecular weightSemantic proximity Read ↗

A transitional hydrolase to glycosynthase mutant by Glu to Asp substitution at the catalytic nucleophile in a retaining glycosidase.Semantic proximity Read ↗

Identification of Functionally Important Amino Acid Residues in Zymomonas mobilis LevansucraseSemantic proximity Read ↗

The role of conserved inulosucrase residues in the reaction and product specificity of Lactobacillus reuteri inulosucrase.Semantic proximity Read ↗

Directed mutation of β-glucanases from probiotics to enhance enzymatic activity, thermal and pH stability.Semantic proximity Read ↗

Molecular insight into regioselectivity of transfructosylation catalyzed by GH68 levansucrase and β-fructofuranosidaseSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.