Production of long-chain levan by a sacC insertional mutant from Bacillus subtilis 327UH.

doi:10.1271/bbb.66.1555

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Article

Production of long-chain levan by a sacC insertional mutant from Bacillus subtilis 327UH.

Bioscience, biotechnology, and biochemistry - 1 Jul 2002

Shida Toshio, Mukaijo Kohsuke, Ishikawa Shu, Yamamoto Hiroki, Sekiguchi Junichi

Abstract excerpt

A hyper extracellular protein producer, Bacillus subtilis 327UH, produced large amounts of levan in a medium containing 20% sucrose, and the yield of levan after 10 hours was more than 60%, when based on the fructose amount of sucrose. After transformation of 327UH with a levanase-deficient 168SC (sacC::Cm(r)) chromosomal DNA, a Cm(r) transformant 327UHSC (sacC::Cm(r) degSU(Hy)) produced 3 times longer levan than...

Topics

Bacillus subtilis
Bacterial Proteins
Culture Media
DNA, Bacterial
Escherichia coli
Fructans
Genes, Bacterial
Glycoside Hydrolases
Mutagenesis, Insertional
Mutation
Plasmids
Sucrose
Transformation, Bacterial

Source

Open in PubMed

Identifiers

PMID: 12224641
DOI: 10.1271/bbb.66.1555

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