Article
Polymerase and hydrolase activities of Bacillus subtilis levansucrase can be separately modulated by site-directed mutagenesis.
The Biochemical journal - 1 Oct 1991
Chambert R, Petit-Glatron M F
Abstract excerpt
The levansucrase (sucrose:2,6-beta-D-fructan 6-beta-D-fructosyltransferase, EC 2.4.1.10) structural gene from a Bacillus subtilis mutant strain displaying a low polymerase activity was sequenced. Only one missense mutation changing Arg331 to His was responsible for this modified catalytic property. From this allele we created new mutations by directed mutagenesis, which modified the charge and polarity of site...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- Blotting, Western
- DNA Fingerprinting
- Fructose
- Genes, Bacterial
- Hexosyltransferases
- Hydrogen-Ion Concentration
- Hydrolases
- Hydrolysis