Article
Allele-specific PCR analysis of p53 codon 249 AGT transversion in liver tissues from patients with viral hepatitis.
International journal of cancer - 27 Sept 1996
Kirby G M, Batist G, Fotouhi-Ardakani N, Nakazawa H, Yamasaki H, Kew M, Cameron R G, Alaoui-Jamali M A
Abstract excerpt
AGG to AGT mutations in codon 249 of the p53 tumor-suppressor gene are frequently observed in hepatocellular carcinomas (HCC) from areas where exposure to aflatoxin B1 (AFB) occurs. We developed a sensitive allele-specific polymerase chain reaction (AS-PCR) assay to detect this point mutation in...
Topics
- Adult
- Aflatoxin B1
- Aged
- Alleles
- Base Sequence
- Codon
- Female
- Genes, p53
- Hepatitis B
- Humans
- Liver
- Liver Neoplasms
- Male
- Middle Aged
- Molecular Sequence Data
- Mozambique
- Point Mutation
- Polymerase Chain Reaction