Article
Characterization of yeast sar1 temperature-sensitive mutants, which are defective in protein transport from the endoplasmic reticulum.
Journal of biochemistry - 1 Aug 1996
Yamanushi T, Hirata A, Oka T, Nakano A
Abstract excerpt
SAR1 encodes a low molecular weight GTPase that is essential in the early process of vesicular transport in the secretory pathway. By random and site-directed mutagenesis of the SAR1 gene, we have obtained three temperature-sensitive mutants, N132I, E112K, and D32G. They all show a defect in tran...
Topics
- Alleles
- Amino Acid Sequence
- Biological Transport, Active
- Endoplasmic Reticulum
- Escherichia coli
- Fungal Proteins
- GTP-Binding Proteins
- Genes, Fungal
- Golgi Apparatus
- Microscopy, Electron
- Molecular Sequence Data
- Monomeric GTP-Binding Proteins
- Mutagenesis
- Mutation
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Temperature
- Vesicular Transport Proteins