Article
Multicopy suppressors of the sly1 temperature-sensitive mutation in the ER-Golgi vesicular transport in Saccharomyces cerevisiae.
Yeast (Chichester, England) - 1 Aug 2001
Kosodo Y, Imai K, Hirata A, Noda Y, Takatsuki A, Adachi H, Yoda K
Abstract excerpt
Saccharomyces cerevisiae Sly1 protein is a member of the Sec1/Munc18-family proteins, which are essential for vesicular trafficking, but their exact biological roles are yet to be determined. A temperature-sensitive sly1 mutant arrests the vesicular transport from the ER to Golgi compartments at 37 degrees C. We screened for multicopy suppressor genes that restore the colony formation of the sly1(ts) mutant to...
Topics
- Amino Acid Sequence
- Carrier Proteins
- Endoplasmic Reticulum
- Epitope Mapping
- Fungal Proteins
- Gene Dosage
- Genes, Fungal
- Genes, Suppressor
- Golgi Apparatus
- Membrane Proteins
- Membrane Transport Proteins
- Molecular Sequence Data
- Munc18 Proteins