Article
Isolation of a tobacco cDNA encoding Sar1 GTPase and analysis of its dominant mutations in vesicular traffic using a yeast complementation system.
Plant & cell physiology - 1 Jun 1998
Takeuchi M, Tada M, Saito C, Yashiroda H, Nakano A
Abstract excerpt
The cDNA clone of NtSAR1, a gene encoding the small GTPase Sar1p which is essential for vesicle formation from the endoplasmic reticulum (ER) membrane in yeast, has been isolated from Nicotiana tabacum BY-2 cells. NtSAR1 as well as AtSAR1 cDNA isolated from Arabidopsis thaliana [d'Enfert et al. (...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Consensus Sequence
- DNA, Complementary
- GTP-Binding Proteins
- Genes, Lethal
- Genetic Complementation Test
- Genotype
- Molecular Sequence Data
- Monomeric GTP-Binding Proteins
- Mutation
- Plants, Toxic
- Recombinant Proteins
- Saccharomyces cerevisiae